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Take 5 ml of the test solution (0.01 g sample dissolved in 1 mL of 95% ethanol) and drop it on thin layer chromatography (microcrystalline cellulose, 0.1 mm), and put it in a container containing 3-methyl-1-butanol/ethanol/water/ In the development tank of ammonia (4:4:2:1) mixture, the solvent front is raised by 10-15 cm. Observation under ultraviolet light after one day and night: there are three yellow spots with Rf between 0.2 and 0.4; the Rf of other spots is 0.6 to 0.8; all spots show yellow fluorescence under ultraviolet light. 6.95% ethanol solution has a maximum absorption peak at 425 nm.
Shaanxi Huike Botanical Development Co., Ltd. a integrated enterprise which is focus on natural products, plant extract related products and services.We mainly focus on pharmaceutical, functional food, Freeze-dried powder,Natural Pigment,Homology of medicine and food,beverages and other business services.
2022-6-16
Email:sales8@huikes.com
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